Small RNA analysis using the Genome Sequencer™ FLX system

H5N1: Whole RNA Virus Sequencing Using the Genome Sequencer FLX System

Introduction The global spread of the deadly H5N1 avian influenza virus among bird populations and its transmission to humans in endemic areas has emphasized the importance of fast and accurate detection methods for influenza virus strain and variant identification. Immunological typing assays are widely used, but they provide limited information for the understanding of, for example, strain ev...

Comprehensive transcriptome analysis with the Genome Sequencer FLX System

Protein isoforms make the transcriptome complex and challenging to resolve. Different forms of a protein may be produced from related genes or may arise from the same gene by alternative splicing. With a combination of long (400–500 bp) 454 SequencingTM reads, dedicated GS De Novo Assembler software and a straightforward protocol using just 200 ng of RNA as sample input, the Genome Sequencer FL...

Transcriptome sequencing with the Genome Sequencer FLX system

of methods. In all methods, experimental success depends upon the removal of ribosomal RNA, as rRNA will be the majority species sequenced if not carefully removed from the total RNA. For all protocols, a control dataset should be established using a high-quality mRNA (for example, rat liver mRNA purchased from a vendor). This With the introduction of next-generation sequencing, transcriptome s...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Predicting CpG Islands and DNA Methlation in the Cow Genome Using DNA Microarray Meta-Analysis and Genome Wide Scanning

DNA methylation is a type of epigenetic changes that directly affects DNA. In mammals, DNA methylation is essential for fetal development and stem cell differentiation and this phenomenon essentially occurs within the CpG islands. In this study, two methods were used to study the DNA methylation profile of cow genome. In the first method, the DNA methylation profile of the differentially expres...